Structural characterization of holo- and apo-myoglobin in the gas phase by ultraviolet photodissociation mass spectrometry.

Structural characterization of holo- and apo-myoglobin in the gas phase by ultraviolet photodissociation mass spectrometry† †Electronic supplementary information (ESI) available: ESI mass spectra of holo- and apo-myoglobin, schematic representation of holo-myoglobin, histograms of HCD and UVPD fragmentation yields of apo-myoglobin, B-factors and solvent accessibilities. See DOI: 10.1039/c4sc03200d Click here for additional data file.

Characterization of hydrogen bonding motifs in proteins: hydrogen elimination monitoring by ultraviolet photodissociation mass spectrometry.

Determination of structure and folding of certain classes of proteins remains intractable by conventional structural characterization strategies and has spurred the development of alternative methodologies. Mass spectrometry-based approaches have a unique capacity to differentiate protein heterogeneity due to the ability to discriminate populations, whether minor or major, featuring modificatio...

Photodissociation of conformer-selected ubiquitin ions reveals site-specific cis/trans isomerization of proline peptide bonds.

Ultraviolet photodissociation (UVPD) of gas-phase proteins has attracted increased attention in recent years. This growing interest is largely based on the fact that, in contrast to slow heating techniques such as collision induced dissociation (CID), the cleavage propensity after absorption of UV light is distributed over the entire protein sequence, which can lead to a very high sequence cove...

ON-LINE SOLID-PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY/PARTICLE BEAM-MASS SPECTROMETRY FOR DEGRADATION STUDIES OF SOME POLAR PESTICIDES IN WATER

An on-line automated method for photodegradation studies of isoproturon, diuron, atrazine, fenitrothion, and metoxuron by means of liquid chromatography/mass spectrometry (LC/MS) with particle beam (PB) interface is described. Surface water samples were first spiked with 50 µg/l of each pesticide and then exposed to the radiation of the medium-pressure mercury lamp. Next, in regular intervals o...

Perchloroethylene Analysis by Chemical Oxidation and Determination of Intermediate Products by Gas Chromatography, Mass Spectrometry

Abstract Background and Objective: Perchloroethylene (PCE) is a chlorinated hydrocarbon used as a solvent in many industrial processes. In contaminated water and soil a great deal of PCE is found. This study aimed to determine the rate of decomposition of PCE occurred after advanced oxidation. Material and Methods: In this descriptive-analytic study conducted (2011) in public health faculty...